anti ly6g pe cy7  (Cytek Biosciences)

Journal: Biology of Sex Differences

Article Title: Sex-based differences in natural killer T cell-mediated protection against diet-induced steatohepatitis in Balb/c mice

doi: 10.1186/s13293-023-00569-w

Figure Lengend Snippet: Neutrophils and macrophage subsets in liver in males and females of Balb/c or CD1d −/− mice after Chow or HFCD feeding. a Neutrophils (Ly6G + ); b MPO immunohistochemistry (20X), red arrows point to MPO-positive cells in male tissue from sections from 6 weeks HFCD-fed Balb/c (WT) or CD1d-KO mice; c macrophages (F4/80 + , CD11b + ); d Ly6C hi macrophages; e Ly6C lo macrophage; or f ratio Ly6C hi /Ly6C lo macrophages were determined by flow cytometry in hepatic tissue of Chow or 6 weeks HFCD-fed Balb/c (WT) or CD1d-KO mice, males or females; g representative F4/80 immunohistochemistry (20x), in 6 weeks HFCD-fed Balb/c (WT) or HFCD-fed CD1d-KO male mice, red arrows indicated hepatic crown-like structures in male tissue sections from HFCD Balb/c (WT) or CD1d-KO mice. Data are expressed as mean ± SEM, n = 4–5, * p < 0.05, ** p < 0.005, *** p < 0.001 vs. corresponding compared group. To compare the means between two particular groups the Student’s t test will be used, and the resulting p value expressed numerically in the figure

Article Snippet: The antibodies used were: mouse CD3 FITC Conjugate #HM3401 (Invitrogen), mouse CD4 APC Conjugate #MCD0405 (Invitrogen), rat Anti-Mouse CD19-PE/CY5.5 #1575-16 (Southern Biotech), rat Anti-Mouse Ly6G/GR-1-PE/CY7 #1900–17 (Southern Biotech), Alexa Fluor 700 Rat Anti-Mouse CD45 #560510 (BD Pharmingen), PerCP-Cy 5.5 Rat Anti-CD11b #550993 (BD Pharmingen), BV605 Rat Anti-Mouse Ly-6C #563011 (BD Horizon), anti-Mouse CD8a PE-Cy7 #60–0081-U025 (TONBO Biosciences), BUV395 Rat Anti-Mouse F4/80 #565614 (BD Horizon), mouse CD1d PBS-57 (an analogue of α-GalCer) (PE-Labeled Tetramer) and mouse CD1d Unloaded (PE-Labeled Tetramer) (NIH Tetramer Core Facility at Emory University).

Techniques: Immunohistochemistry, Flow Cytometry